Rabbit anti-Human MPO Polyclonal Antibody | anti-MPO antibody

MPO, CT (MPO, Myeloperoxidase, 89kD myeloperoxidase, 84kD myeloperoxidase, Myeloperoxidase light chain, Myeloperoxidase heavy chain)

Cat. Num. AAA641141

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MPO; Polyclonal Antibody; CT (MPO; Myeloperoxidase; 89kD myeloperoxidase; 84kD myeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain); Anti -MPO; anti-MPO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MPO antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: MPO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-575 amino acids from the C-terminal region of human MPO.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using MBS641141. This demonstrates that MBS641141 detected the MPO protein (arrow).)

Related Product Information for anti-MPO antibody

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq].

Product Categories/Family for anti-MPO antibody

Antibodies; Abs to Enzymes; Myeloperoxidase

NCBI and Uniprot Product Information

• NCBI GI #: 4557759
• NCBI GeneID: 4353
• NCBI Accession #: NP_000241.1
• NCBI GenBank Nucleotide #: NM_000250.1
• UniProt Accession #: P05164; Q14862; Q4PJH5; Q9UCL7; A1L4B8
• Molecular Weight: 83,869 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.11.2.2

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; aging; negative regulation of apoptosis

Disease: Myeloperoxidase Deficiency; Alzheimer Disease