Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGCD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SGCD transfected 293T cell lysate )
Mouse anti-Human SGCD Monoclonal Antibody | anti-SGCD antibody
SGCD antibody
Gene Names
SGCD; SGD; DAGD; 35DAG; CMD1L; SGCDP; SG-delta
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
SGCD; Monoclonal Antibody; SGCD antibody; Delta-sarcoglycan; 35DAG; CMD1L; DAGD; SG-delta; SGCDP; SGD; anti-SGCD antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1b,k
Clone Number
AT19G8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
289
Applicable Applications for anti-SGCD antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended starting dilution is 1:1000.
Antigen Species
Human
Immunogen
Recombinant human SGCD (57-289aa) purified from E. coli
Clone Note
Anti-human SGCD mAb, clone AT19G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SGCD protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGCD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SGCD transfected 293T cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGCD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SGCD transfected 293T cell lysate )
Related Product Information for anti-SGCD antibody
SGCD is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. It is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Product Categories/Family for anti-SGCD antibody
References
Thompson T.G., et al. (2000) J. Biol. Chem. 148: 115-126; Entrez Gene: SGCD sarcoglycan, delta;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,750 Da
NCBI Official Full Name
delta-sarcoglycan isoform 3
NCBI Official Synonym Full Names
sarcoglycan delta
NCBI Official Symbol
SGCD
NCBI Official Synonym Symbols
SGD; DAGD; 35DAG; CMD1L; SGCDP; SG-delta
NCBI Protein Information
delta-sarcoglycan
UniProt Protein Name
Delta-sarcoglycan
Protein Family
UniProt Gene Name
SGCD
UniProt Synonym Gene Names
Delta-SG; 35DAG
NCBI Description
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Research Articles on SGCD
Similar Products
Product Notes
The SGCD sgcd (Catalog #AAA200310) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SGCD antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SGCD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the SGCD sgcd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGCD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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