Human microfibrillar associated protein 5 ELISA Kit | MFAP5 elisa kit

Human Microfibrillar-associated protein 5, MFAP5 ELISA Kit

Cat. Num. AAA933375

• Gene Names: MFAP5; MP25; MAGP2; MAGP-2; MFAP-5
• Reactivity: Human
• Synonyms: microfibrillar associated protein 5; Human Microfibrillar-associated protein 5; MFAP5 ELISA Kit; Human Microfibrillar-associated protein 5 (MFAP5) ELISA kit; MAGP2; MP25; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; MFAP5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MFAP5. No significant cross-reactivity or interference between human MFAP5 and analogues was observed.
• Sequence Length: 173

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: 0.039 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFAP5 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFAP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4505089
• NCBI GeneID: 8076
• NCBI Accession #: NP_003471.1
• NCBI GenBank Nucleotide #: NM_003480.3
• UniProt Accession #: Q13361; B0AZL6; D3DUV1
• Molecular Weight: 19,612 Da
• NCBI Official Full Name: microfibrillar-associated protein 5 isoform a
• NCBI Official Synonym Full Names: microfibrillar associated protein 5
• NCBI Official Symbol: MFAP5
• NCBI Official Synonym Symbols: MP25; MAGP2; MAGP-2; MFAP-5
• NCBI Protein Information: microfibrillar-associated protein 5; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2
• UniProt Protein Name: Microfibrillar-associated protein 5
• Protein Family: Microfibrillar-associated protein
• UniProt Gene Name: MFAP5
• UniProt Synonym Gene Names: MAGP2; MFAP-5; MAGP-2
• UniProt Entry Name: MFAP5_HUMAN

NCBI Description

This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12p13.1-p12.3

Cellular Component: extracellular region; microfibril

Molecular Function: extracellular matrix structural constituent

Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis

Disease: Aortic Aneurysm, Familial Thoracic 9