Typical Testing Data/Standard Curve (for reference only)
Human Lymphoblastic Leukemia Derived Sequence 1 (LYL1) ELISA Kit | LYL1 elisa kit
Human Lymphoblastic Leukemia Derived Sequence 1 (LYL1) ELISA Kit
Gene Names
LYL1; bHLHa18
Reactivity
Human
Synonyms
Lymphoblastic Leukemia Derived Sequence 1 (LYL1); Human Lymphoblastic Leukemia Derived Sequence 1 (LYL1) ELISA Kit; bHLHa18; Class A basic helix-loop-helix protein 18; Lymphoblastic leukemia-derived sequence 1; LYL1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Lymphoblastic Leukemia Derived Sequence 1 (LYL1).
No significant cross-reactivity or interference between Lymphoblastic Leukemia Derived Sequence 1 (LYL1) and analogues was observed.
No significant cross-reactivity or interference between Lymphoblastic Leukemia Derived Sequence 1 (LYL1) and analogues was observed.
Sequence Length
280
Assay Type
Double-antibody Sandwich
Sample
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Dectection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.055ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphoblastic Leukemia Derived Sequence 1 (LYL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphoblastic Leukemia Derived Sequence 1 (LYL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphoblastic Leukemia Derived Sequence 1 (LYL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LYL1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphoblastic Leukemia Derived Sequence 1 (LYL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphoblastic Leukemia Derived Sequence 1 (LYL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphoblastic Leukemia Derived Sequence 1 (LYL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphoblastic Leukemia Derived Sequence 1 (LYL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LYL1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein lyl-1
NCBI Official Synonym Full Names
LYL1 basic helix-loop-helix family member
NCBI Official Symbol
LYL1
NCBI Official Synonym Symbols
bHLHa18
NCBI Protein Information
protein lyl-1
UniProt Protein Name
Protein lyl-1
Protein Family
UniProt Gene Name
LYL1
UniProt Synonym Gene Names
BHLHA18; bHLHa18
UniProt Entry Name
LYL1_HUMAN
NCBI Description
This gene represents a basic helix-loop-helix transcription factor. The encoded protein may play roles in blood vessel maturation and hematopoeisis. A translocation between this locus and the T cell receptor beta locus (GeneID 6957) on chromosome 7 has been associated with acute lymphoblastic leukemia. [provided by RefSeq, Sep 2010]
Uniprot Description
LYL1: A chromosomal aberration involving LYL1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(7;19)(q35;p13) with TCRB.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleus
Molecular Function: protein dimerization activity; protein binding; DNA binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; B cell differentiation; blood vessel maturation
Research Articles on LYL1
Similar Products
Product Notes
The Human LYL1 lyl1 (Catalog #AAA2122038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2122038 ELISA Kit recognizes Human LYL1. It is sometimes possible for the material contained within the vial of "Lymphoblastic Leukemia Derived Sequence 1 (LYL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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