Human Vimentin (VIM) ELISA Kit | VIM elisa kit

Human Vimentin (VIM) ELISA Kit

Cat. Num. AAA2024120

• Gene Names: VIM; HEL113; CTRCT30
• Reactivity: Human
• Synonyms: Vimentin (VIM); Human Vimentin (VIM) ELISA Kit; VIM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of VGLL1. No significant cross-reactivity or interference between VGLL1 and analogues was observed.
• Sequence Length: 466

• Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5,000pg/mL
• Sensitivity: 32pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGLL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGLL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VIM elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VGLL1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VGLL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VGLL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGLL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VGLL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VIM elisa kit

Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.3
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
• Molecular Weight: 53,652 Da
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Official Synonym Symbols: HEL113; CTRCT30
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis

Disease: Cataract 30