Human LILRB2 ELISA Kit | LILRB2 elisa kit

Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2) ELISA Kit

Cat. Num. AAA2515505

• Gene Names: LILRB2; ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• Reactivity: Human
• Synonyms: LILRB2; Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2) ELISA Kit; LILRB2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human LILRB2. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed.
• Sequence Length: 510

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LILRB2 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LILRB2 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LILRB2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LILRB2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LILRB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LILRB2. You can calculate the concentration of LILRB2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 509155854
• NCBI GeneID: 10288
• NCBI Accession #: NP_001265334.1
• NCBI GenBank Nucleotide #: NM_001278405.1
• UniProt Accession #: Q8N423; O75017; Q8NHJ7; Q8NHJ8; A8MU67; C9JF29
• Molecular Weight: 51,959 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 2 isoform 4
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
• NCBI Official Symbol: LILRB2
• NCBI Official Synonym Symbols: ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 2; CD85 antigen-like family member D; Ig-like transcript 4; monocyte/macrophage immunoglobulin-like receptor 10
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 2
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB2
• UniProt Synonym Gene Names: ILT4; LIR2; MIR10; LIR-2; Leukocyte immunoglobulin-like receptor 2; ILT-4; MIR-10
• UniProt Entry Name: LIRB2_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB2: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; MHC class I protein binding; receptor activity; cell adhesion molecule binding; protein phosphatase 1 binding; inhibitory MHC class I receptor activity

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; negative regulation of calcium ion transport; regulation of immune response; positive regulation of regulatory T cell differentiation; negative regulation of immune response; positive regulation of interleukin-6 production; signal transduction; positive regulation of tolerance induction; heterotypic cell-cell adhesion; cell surface receptor linked signal transduction; negative regulation of T cell proliferation; cell-cell signaling; negative regulation of antigen processing and presentation; cellular defense response; Fc receptor mediated inhibitory signaling pathway; positive regulation of T cell proliferation; immune response; positive regulation of T cell tolerance induction