SDS-Page
ATPIF1 recombinant protein
ATPIF1, 26-106aa, Human, His tag, E Coli
Gene Names
ATP5IF1; IP; ATPI; ATPIP; ATPIF1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
ATPIF1; 26-106aa; Human; His tag; E Coli; ATPase inhibitor mitochondrial; ATPI; ATPIP; IP; ATPase Inhibitory Factor 1; ATPIF1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD
Sequence Length
106
Applicable Applications for ATPIF1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for ATPIF1 recombinant protein
ATPase inhibitory factor 1, also known as ATPIF1, binds to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, via interference of the beta and gamma subunit interaction, ATPIF1 regulates the activity of the F1 (the hydrophilic catalytic core), F0 (the membrane embedded protein channel) ATPase. The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis. Recombinant human ATPIF1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ATPIF1 recombinant protein
References
Sanchez Cenizo L., et al. (2010) J Biol Chem. 285: 25308-13.; Cortes Hernandez P., et al. (2005) Biochem Biophys Res Commun. 330: 844-849.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12.2 kDa (106aa), confirmed by MALDI-TOF
NCBI Official Full Name
ATPase inhibitor, mitochondrial isoform 1
NCBI Official Synonym Full Names
ATP synthase inhibitory factor subunit 1
NCBI Official Symbol
ATP5IF1
NCBI Official Synonym Symbols
IP; ATPI; ATPIP; ATPIF1
NCBI Protein Information
ATPase inhibitor, mitochondrial
UniProt Protein Name
ATPase inhibitor, mitochondrial
Protein Family
UniProt Gene Name
ATPIF1
UniProt Synonym Gene Names
ATPI; IF(1); IF1
NCBI Description
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Research Articles on ATPIF1
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Product Notes
The ATPIF1 atpif1 (Catalog #AAA204583) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ATPIF1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ATPIF1 atpif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD. It is sometimes possible for the material contained within the vial of "ATPIF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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