Rabbit anti-Human, Mouse Epo-R Polyclonal Antibody | anti-Epo-R antibody

Epo-R (Phospho-Tyr368) Antibody

Cat. Num. AAA9401346

• Gene Names: EPOR; EPO-R
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Epo-R; Polyclonal Antibody; Epo-R (Phospho-Tyr368) Antibody; EPO R; EPO-R; epor; anti-Epo-R antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Epo-R only when phosphorylated at tyrosine 368.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 508

• Applicable Applications for anti-Epo-R antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Tyrosine 368 (D-T-Y(p)-L-V) derived from Human Epo-R.
• Modification: Phospho-Tyr368
• Target Name: Epo-R
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from K562 tissue using Epo-R (Phospho-Tyr368) antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-Epo-R antibody

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.Funakoshi-Tago M, et al. (2010) J Biol Chem 285, 5296-307 Sulahian R, Cleaver O, Huang LJ (2009) Blood 113, 5287-97 Zhang YL, et al. (2009) Mol Cell 33, 266-74

Product Categories/Family for anti-Epo-R antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NM_000121.3
• UniProt Accession #: P19235; Q15443; Q2M205; B2RCG4
• Molecular Weight: 35,809 Da
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; brain development; decidualization; signal transduction

Disease: Erythrocytosis, Familial, 1