Human LILRB2 ELISA Kit | LILRB2 elisa kit

Human LILRB2 ELISA Kit

Cat. Num. AAA1753901

• Gene Names: LILRB2; ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• Reactivity: Human
• Synonyms: LILRB2; Human LILRB2 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 2; LIR-2; Leukocyte immunoglobulin-like receptor 2; CD85 antigen-like family member D; Immunoglobulin-like transcript 4; ILT-4; Monocyte/macrophage immunoglobulin-like receptor 10; MIR-10; CD85d; ILT4; LIR2; MIR10; leukocyte immunoglobulin like receptor B2; LILRB2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human LILRB2

• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <50pg/ml
• Capture/Detection Antibodies: Capture antibody- a monoclonal antibody from mouse|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000; O.D.|0.055|0.125|0.169|0.299|0.545|0.956|1.437|2.064
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|384|2313|8445|; Standard deviation|17.28|131.84|388.47|; CV(%)|4.5%|5.7%|4.6%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|492|2436|9458; Standard deviation|25.09|143.72|699.89; CV(%)|5.1%|5.9%|7.4%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 384|401|375|392|388|9.61|2.4%; Sample 2: 2313|1997|2156|1982|2112|134.59|6.3%; Sample 3: 8445|7435|7826|7234|7735|461.89|5.9%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q22-V461
• Anticoagulant: heparin, EDTA or citrate
• Principle: Human LILRB2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LILRB2 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: Q22-V461) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LILRB2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LILRB2 amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human LILRB2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. We recommended that each human LILRB2 standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human LILRB2 ELISA Kit Standard Curve)

Related Product Information for LILRB2 elisa kit

Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Product Categories/Family for LILRB2 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 125988400
• NCBI GeneID: 10288
• NCBI Accession #: NP_005865.2
• NCBI GenBank Nucleotide #: NM_005874.3
• UniProt Accession #: Q8N423; O75017; Q8NHJ7; Q8NHJ8
• Molecular Weight: 65,039 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 2 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
• NCBI Official Symbol: LILRB2
• NCBI Official Synonym Symbols: ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 2; Ig-like transcript 4; CD85 antigen-like family member D; monocyte/macrophage immunoglobulin-like receptor 10
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 2
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB2
• UniProt Synonym Gene Names: ILT4; LIR2; MIR10; LIR-2; Leukocyte immunoglobulin-like receptor 2; ILT-4; MIR-10
• UniProt Entry Name: LIRB2_HUMAN