Rabbit anti-Human, Mouse ADH4 Polyclonal Antibody | anti-ADH4 antibody

ADH4, CT (ADH4, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain)

Cat. Num. AAA6001380

• Gene Names: ADH4; NRC465; ZRG5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ADH4; Polyclonal Antibody; CT (ADH4; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain); Anti -ADH4; anti-ADH4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ADH4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human ADH4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADH4 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ADH4 antibody detected the ADH4 protein (arrow).)

Western Blot (WB)

(Western blot analysis of ADH4 (arrow) using rabbit polyclonal ADH4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.)

Immunohistochemistry (IHC)

(ADH4 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH4 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADH4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADH4 antibody

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Product Categories/Family for anti-ADH4 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 269970305
• NCBI GeneID: 852636
• NCBI Accession #: NP_011258.2
• NCBI GenBank Nucleotide #: NM_001181122.1
• UniProt Accession #: P10127; D6VV79
• Molecular Weight: 41,142 Da
• NCBI Official Full Name: alcohol dehydrogenase ADH4
• NCBI Official Symbol: ADH4
• NCBI Official Synonym Symbols: NRC465; ZRG5
• NCBI Protein Information: alcohol dehydrogenase ADH4
• UniProt Protein Name: Alcohol dehydrogenase 4
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH4
• UniProt Synonym Gene Names: ZRG5; ADHIV
• UniProt Entry Name: ADH4_YEAST

Uniprot Description

Function: Reduces acetaldehyde to ethanol during glucose fermentation. Specific for ethanol. Shows drastically reduced activity towards primary alcohols from 4 carbon atoms upward. Isomers of aliphatic alcohol, as well as secondary alcohols and glycerol are not used at all. Ref.13

Catalytic activity: An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor: Zinc. May bind iron when zinc levels are limiting. Ref.5

Enzyme regulation: Inhibited by EDTA. Ref.5

Subunit structure: Homodimer. Ref.5

Subcellular location: Mitochondrion Ref.8.

Induction: Induced by transcription factor ZAP1 in response to zinc deficiency. Ref.5 Ref.6 Ref.7 Ref.10 Ref.12 Ref.13

Miscellaneous: While ADH4 is expressed at only low levels in laboratory strains, it is often highly expressed in brewing strains.Present with 125 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the iron-containing alcohol dehydrogenase family.

Biophysicochemical propertiesKinetic parameters:KM=59 mM for NAD Ref.5KM=122 µM for NADHKM=2.83 mM for acetaldehydeKM=16.7 mM for ethanolpH dependence:Optimum pH is 8.3.

Sequence caution: The sequence CAA64131.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96976.1 differs from that shown. Reason: Erroneous initiation.