Rabbit SPAM1 Polyclonal Antibody | anti-SPAM1 antibody

SPAM1 Polyclonal Antibody

Cat. Num. AAA3008163

• Gene Names: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SPAM1; Polyclonal Antibody; SPAM1 Polyclonal Antibody; Hyaluronidase PH-20; Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20; HYAL3; PH20; anti-SPAM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPAM1 polyclonal antibody detects endogenous levels of SPAM1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 509

• Applicable Applications for anti-SPAM1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human SPAM1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of SPAM1 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells, using SPAM1 polyclonal antibody)

Related Product Information for anti-SPAM1 antibody

Hyaluronidases (HAases or HYALs) are a family of lysosomal enzymes that are crucial for the spread of bacterial infections and of toxins present in a variety of venoms. HYALs may also be involved in the progression of cancer. In humans, six members of the hyaluronidase family have been identified. These proteins are significant in the degradation of hyaluronic acid (HA), which is present in body fluids, tissues and the extracellular matrix of vertebrate tissues. HA keeps tissues hydrated, maintains osmotic balance and promotes cell proliferation, differentiation and metastasis. HA is also an important structural component of cartilage and other tissues and acts as a lubricant in joints. SPAM1 (sperm adhesion molecule 1), also designated Hyal-PH20 or sperm surface protein PH-20, was formerly referred to as HYAL1 (HYA1). The current nomenclature references the functional hyaluronidase activity of SPAM1 to permit acrosome-intact sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte. SPAM1 is also implicated in intracellular signaling and zona pellucida binding. SPAM1 is found in the epididymis and in testis, more specifically in plasma and acrosomal membranes of sperm. SPAM1 is a structurally unique hyaluronidase in that it is a GPI-anchored protein.

NCBI and Uniprot Product Information

• NCBI GI #: 585673
• NCBI GeneID: 6677
• UniProt Accession #: P38567
• Molecular Weight: ~ 47kDa
• NCBI Official Full Name: Hyaluronidase PH-20
• NCBI Official Synonym Full Names: sperm adhesion molecule 1
• NCBI Official Symbol: SPAM1
• NCBI Official Synonym Symbols: HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• NCBI Protein Information: hyaluronidase PH-20
• UniProt Protein Name: Hyaluronidase PH-20
• Protein Family: Hyaluronidase
• UniProt Gene Name: SPAM1
• UniProt Synonym Gene Names: HYAL3; PH20; Hyal-PH20
• UniProt Entry Name: HYALP_HUMAN

NCBI Description

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction