Typical Testing Data/Standard Curve (for reference only)
(Human Kallistatin/Serpina4 PicoKine ELISA Kit standard curve )
Human Kallistatin/Serpina4 ELISA Kit | SERPINA4 elisa kit
Human Kallistatin/Serpina4 PicoKine ELISA Kit
Gene Names
SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin
Reactivity
Human
Synonyms
Kallistatin/Serpina4; Human Kallistatin/Serpina4 PicoKine ELISA Kit; Kallistatin; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 4; KAIN_HUMAN; KAL; Kallikrein binding protein; Kallikrein inhibitor; KBP; KLST; KST; MGC108582; Peptidase inhibitor 4; PI-4; PI4; Protease inhibitor 4 (kallistatin); Protease inhibitor 4; Serine (or cysteine) proteinase inhibitor; clade A (alpha 1 antiproteinase; Serpin A4; Serpin peptidase inhibitor clade A member 4; Serpin peptidase inhibitor; SERPINA4; Tissue kallistatin inhibitor; SERPINA4 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Kallistatin
Sequence Length
427
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Kallistatin/Serpina4 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Kallistatin/Serpina4 PicoKine ELISA Kit standard curve )
Related Product Information for SERPINA4 elisa kit
Principle of the assay: human Kallistatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallistatin has been precoated onto 96-well plates. Standards(NSO, Q21-P427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallistatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallistatin amount of sample captured in plate.
Background: Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.
Background: Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,542 Da
NCBI Official Full Name
Kallistatin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
NCBI Official Symbol
SERPINA4
NCBI Official Synonym Symbols
KAL; KST; PI4; KLST; PI-4; kallistatin
NCBI Protein Information
kallistatin; serpin A4; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
UniProt Protein Name
Kallistatin
Protein Family
UniProt Gene Name
SERPINA4
UniProt Synonym Gene Names
KST; PI4; PI-4
UniProt Entry Name
KAIN_HUMAN
Uniprot Description
SERPINA4: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on SERPINA4
Similar Products
Product Notes
The Human SERPINA4 serpina4 (Catalog #AAA177176) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177176 ELISA Kit recognizes Human SERPINA4. It is sometimes possible for the material contained within the vial of "Kallistatin/Serpina4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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