Human Kallistatin/Serpina4 ELISA Kit | SERPINA4 elisa kit

Human Kallistatin/Serpina4 Quick ELISA Kit

Cat. Num. AAA1753933

• Gene Names: SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin
• Reactivity: Human
• Synonyms: Kallistatin/Serpina4; Human Kallistatin/Serpina4 Quick ELISA Kit; Serpin A4/Kallistatin|antitrypsin); member 4; KAL; Kallistatin; KLST; KSTKallikrein inhibitor; PI-4; PI4Peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; Serpin A4; serpin peptidase inhibitor; antitrypsin); SERPINA4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human SERPINA4

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.010|0.132|0.225|0.431|0.753|1.438|2.208|2.404
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|251|1712|4269|; Standard deviation|12.05|94.16|204.91|; CV(%)|4.8%|5.5%|4.8%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|229|1563|4117; Standard deviation|12.37|90.65|251.14; CV(%)|5.4%|5.8%|6.1%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 251|266|244|301|266|16.20|6.1%; Sample 2: 1712|1764|1650|1844|1743|50.53|2.9%; Sample 3: 4369|4597|4696|4723|4596|133.29|2.9%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-P427
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human Kallistatin/Serpina4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human SERPINA4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human SERPINA4 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-P427.; ; To measure Human SERPINA4, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human SERPINA4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SERPINA4 in the sample.
• Assay Procedure Step 1: 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for SERPINA4 elisa kit

Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32. 13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Product Categories/Family for SERPINA4 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 573459745
• NCBI GeneID: 5267
• NCBI Accession #: NP_001275962.1
• NCBI GenBank Nucleotide #: NM_001289033.1
• UniProt Accession #: P29622; Q53XB5; Q86TR9; Q96BZ5
• Molecular Weight: 48,542 Da
• NCBI Official Full Name: kallistatin isoform 2
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
• NCBI Official Symbol: SERPINA4
• NCBI Official Synonym Symbols: KAL; KST; PI4; KLST; PI-4; kallistatin
• NCBI Protein Information: kallistatin; serpin A4; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
• UniProt Protein Name: Kallistatin
• Protein Family: Kallistatin
• UniProt Gene Name: SERPINA4
• UniProt Synonym Gene Names: KST; PI4; PI-4
• UniProt Entry Name: KAIN_HUMAN

Uniprot Description

SERPINA4: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity