Rabbit anti-Human CFP Polyclonal Antibody | anti-CFP antibody

CFP, ID (CFP, PFC, Properdin, Complement factor P) (MaxLight 750)

Cat. Num. AAA6285412

• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CFP; Polyclonal Antibody; ID (CFP; PFC; Properdin; Complement factor P) (MaxLight 750); Complement factor P; anti-CFP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CFP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-CFP antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 197-223 in the central region of human CFP, UniProt Accession #P27918.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS644545. CFP (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of CEM cells using MBS644545 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CFP antibody

This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3-and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections.

Product Categories/Family for anti-CFP antibody

Antibodies; Complement

References

1. eitsonen, S., et al. Mol. Immunol. 47(6):1334-1336(2010). 2. Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). 3. Wolf-Schnurrbusch, U.E., et al. Retina (Philadelphia, Pa.) 29(7):966-973(2009). 4. Keslar, K., et al. Transplantation 86(9):1319-1321(2008). 5. Hartmann, S., et al. J. Biol. Chem. 275(37):28569-28574(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 355757320
• UniProt Accession #: P27918; O15134; O15135; O15136; O75826
• Molecular Weight: 51,276 Da
• NCBI Official Full Name: Complement factor P
• UniProt Protein Name: Properdin
• Protein Family: Properdin
• UniProt Gene Name: CFP
• UniProt Synonym Gene Names: PFC
• UniProt Entry Name: PROP_HUMAN

Uniprot Description

CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; defense response to bacterium; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; post-translational protein modification; complement activation

Disease: Properdin Deficiency, X-linked