Human IL-15 ELISA Kit | IL-15 elisa kit

Human IL-15 PicoKine Fast ELISA Kit

Cat. Num. AAA1752721

• Gene Names: IL15; IL-15
• Reactivity: Human
• Synonyms: IL-15; Human IL-15 PicoKine Fast ELISA Kit; Human IL-15; Interleukin-15; IL15; IL-15 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL15
• Sequence Length: 162

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <3pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-15 elisa kit

Principle of the Assay: The Picokine™ Human IL15 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL15 with a 96-well strip plate that is pre-coated with antibody specific for IL15. The detection antibody is a biotinylated antibody specific for IL15. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IL15 with immunogen: Expression system for standard: E.coli; Immunogen sequence: N49-S162. The kit is analytically validated with ready to use reagents. To measure Human IL15, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human IL15 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL15 in the sample.!!Background/Introduction: Interleukin(IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2(IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 10835153
• NCBI GeneID: 3600
• NCBI Accession #: NP_000576.1
• NCBI GenBank Nucleotide #: NP_000576.1
• UniProt Accession #: P40933
• NCBI Official Full Name: interleukin-15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• UniProt Gene Name: IL15
• UniProt Synonym Gene Names: IL-15
• UniProt Entry Name: IL15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]