Rabbit anti-Human YBX2 Polyclonal Antibody | anti-YBX2 antibody

YBX2, CT (Y-box-binding Protein 2, Germ Cell-specific Y-box-binding Protein, Contrin, MSY2 Homolog, DNA-binding Protein C, Dbpc) (AP)

Cat. Num. AAA6506377

• Gene Names: YBX2; DBPC; MSY2; CSDA3; CONTRIN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: YBX2; Polyclonal Antibody; CT (Y-box-binding Protein 2; Germ Cell-specific Y-box-binding Protein; Contrin; MSY2 Homolog; DNA-binding Protein C; Dbpc) (AP); anti-YBX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human YBX2.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-YBX2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human YBX2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of YBX2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). YBX2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with YBX2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-YBX2 antibody

Antibodies; Transcription Factors

References

1. Kohno, Y., Br. J. Cancer 94(5), 710-716 (2006). 2. Yoshida, T., Biochim. Biophys. Acta 1759(1-2), 80-88 (2006). 3. Tekur, S., J. Androl. 20(1), 135-144 (1999). 4. Gu, W., Biol. Reprod. 59(5), 1266-1274 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 156415990
• NCBI GeneID: 51087
• NCBI Accession #: NP_057066
• NCBI GenBank Nucleotide #: NM_015982.3
• UniProt Accession #: Q9Y2T7; Q8N4P0; D3DTP1
• Molecular Weight: 38,518 Da
• NCBI Official Full Name: Y-box-binding protein 2
• NCBI Official Synonym Full Names: Y box binding protein 2
• NCBI Official Symbol: YBX2
• NCBI Official Synonym Symbols: DBPC; MSY2; CSDA3; CONTRIN
• NCBI Protein Information: Y-box-binding protein 2; MSY2 homolog; DNA-binding protein C; germ cell specific Y-box binding protein; germ cell-specific Y-box-binding protein
• UniProt Protein Name: Y-box-binding protein 2
• Protein Family: Y-box-binding protein
• UniProt Gene Name: YBX2
• UniProt Synonym Gene Names: CSDA3; MSY2; Dbpc
• UniProt Entry Name: YBOX2_HUMAN

NCBI Description

This gene encodes a nucleic acid binding protein which is highly expressed in germ cells. The encoded protein binds to a Y-box element in the promoters of certain genes but also binds to mRNA transcribed from these genes. Pseudogenes for this gene are located on chromosome 10 and 15. [provided by RefSeq, Feb 2012]

Uniprot Description

YBX2: Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: polysome; cytoplasm; nucleolus; nucleus

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding; DNA binding; translation regulator activity; chromatin binding; lipid binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; translational attenuation; mRNA stabilization; negative regulation of translation; spermatogenesis; negative regulation of binding; oocyte development; spermatid development