Typical Testing Data/Standard Curve (for reference only)
Human Fibroblast growth factor 21 (FGF-21) ELISA Kit | FGF-21 elisa kit
Fibroblast growth factor 21 (FGF-21): Human (ELISA)
Reactivity
Human
Synonyms
Fibroblast growth factor 21 (FGF-21); Fibroblast growth factor 21 (FGF-21): Human (ELISA); Human FGF-21; FGF-21 elisa kit
Reactivity
Human
Specificity
Cross Reactivity of recombinant proteins
Sequence Length
209
Samples
Serum, plasma and cell culture supernate samples
Assay Type
Quantitative Sandwich
Sensitivity
30 pg/ml
Intra-assay Precision
Two samples of known concentration were tested 12 times on one plate.
Inter-assay Precision
Two samples of known concentration were tested in 10 separate assays.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGF-21 elisa kit
Intended Uses: This human FGF-21 ELISA kit is designed for quantification of human FGF- 21 in serum, plasma and cell culture supernate samples.
Principle of the Assay: This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-21. Standards and samples are pipetted into the wells and any human FGF-21 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-21 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-21 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-21, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic beta-cells[3,4]. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from dietinduced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].
Principle of the Assay: This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-21. Standards and samples are pipetted into the wells and any human FGF-21 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-21 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-21 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-21, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic beta-cells[3,4]. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from dietinduced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].
References
1. Stressed Liver and Muscle Call on Adipocytes with FGF21. Luo Y, McKeehan WL. Front Endocrinol 2013 18;4:194
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
Protein Family
Similar Products
Product Notes
The Human FGF-21 (Catalog #AAA398051) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA398051 ELISA Kit recognizes Human FGF-21. It is sometimes possible for the material contained within the vial of "Fibroblast growth factor 21 (FGF-21), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
