SDS-Page
Fibroblast growth factor 21 (FGF-21) Recombinant Protein | FGF21 recombinant protein
Fibroblast growth factor 21 (FGF-21): Mouse, Tagless (Recombinant protein)
Applications
ELISA, Western Blot
Purity
>95%; SDS PAGE to determine purity of the protein
Synonyms
Fibroblast growth factor 21 (FGF-21); Fibroblast growth factor 21 (FGF-21): Mouse; Tagless (Recombinant protein); Fibroblast growth factor 21; tagless; Recombinant FGF-21 (Mouse); FGF21 recombinant protein
Host
E Coli
Purity/Purification
>95%; SDS PAGE to determine purity of the protein
Form/Format
Lyophilized in 1mg/mL in PBS
Sequence
GA AY PIPDSSPLLQ FGGQVRQRYL YTDDDQDTEA HLEIREDGTV VGAAHRSPES LLELKALKPG VIQILGVKAS RFLCQQPDGA LYGSPHFDPE ACSFRELLLE DGYNVYQSEA HGLPLRLPQK DSPNQDATSW GPVRFLPMPG LLHEPQDQAG FLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
Sequence Length
209
Applicable Applications for FGF21 recombinant protein
ELISA (EIA), Western Blot (WB)
Species
Mouse
Endotoxin Level
<0.2EU/ug
Reconstitution
Add sterile deionized water to prepare a working stock solution of approximately1mg/mL and let the lyophilized pellet dissolve completely.
Quality Control Test
BCA to determine quantity of the protein.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot reconstituted protein and store at -80 degree C. Avoid repeated freezing /thawing cycles.
SDS-Page
SDS-Page
Testing Data
Testing Data
Related Product Information for FGF21 recombinant protein
Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic beta-cells. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from diet- induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance. Adiponectin has been shown to be critical in mediating the beneficial metabolic actions of FGF-21.
Total 184 AA Mw: 20kDa (calculated). N-terminal His-tag removed, 2 extra AA left.
Total 184 AA Mw: 20kDa (calculated). N-terminal His-tag removed, 2 extra AA left.
References
1. Stressed Liver and Muscle Call on Adipocytes with FGF21. Luo Y, McKeehan WL. Front Endocrinol 2013 18;4:194
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
[1] Nishimura T, Nakatake Y, Konishi M, Itoh N: Identification of a novel FGF, FGF -21, preferentially expressed inthe liver. Biochim Biophys Acta1492:203-206, 2000
[2] Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627-1635, 2005
[3]Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor21.Endocrinology.148(2):774-781. 2007
[4] Hiroshi Kurosu et al. Tissue-speci c Expression of betaKlotho and Fibroblast Growth Factor (FGF) Receptor IsoformsDetermines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687-26695. 2007
[5] Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad SciUSA 104: 7432-7437, 2007.
[6]Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates asubunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USAVol. 83, pp. 5968-5972, August 1986
[7]Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathwayduring Xenopus mesoderm induction. Development 128, 35-44 (2001)
[8]Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast GrowthFactor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical VeinEndothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp.
21237-21245, 2002
[9]Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-MediatedSuppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875-3891. 2002[10] Dostálová I, Haluzíková D, Haluzík M.Fibroblast Growth Factor 21: A Novel Metabolic Regulator With PotentialTherapeutic Properties in Obesity/Type 2 Diabetes Mellitus. Physiol. Res. 58: 1-7, 2009
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
[1] Nishimura T, Nakatake Y, Konishi M, Itoh N: Identification of a novel FGF, FGF -21, preferentially expressed inthe liver. Biochim Biophys Acta1492:203-206, 2000
[2] Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627-1635, 2005
[3]Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor21.Endocrinology.148(2):774-781. 2007
[4] Hiroshi Kurosu et al. Tissue-speci c Expression of betaKlotho and Fibroblast Growth Factor (FGF) Receptor IsoformsDetermines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687-26695. 2007
[5] Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad SciUSA 104: 7432-7437, 2007.
[6]Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates asubunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USAVol. 83, pp. 5968-5972, August 1986
[7]Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathwayduring Xenopus mesoderm induction. Development 128, 35-44 (2001)
[8]Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast GrowthFactor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical VeinEndothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp.
21237-21245, 2002
[9]Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-MediatedSuppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875-3891. 2002[10] Dostálová I, Haluzíková D, Haluzík M.Fibroblast Growth Factor 21: A Novel Metabolic Regulator With PotentialTherapeutic Properties in Obesity/Type 2 Diabetes Mellitus. Physiol. Res. 58: 1-7, 2009
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20kDa
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
Protein Family
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Product Notes
The FGF21 (Catalog #AAA398088) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fibroblast growth factor 21 (FGF-21) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the FGF21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GA AY PIPDSSPLLQ FGGQVRQRYL YTDDDQDTEA HLEIREDGTV VGAAHRSPES LLELKALKPG VIQILGVKAS RFLCQQPDGA LYGSPHFDPE ACSFRELLLE DGYNVYQSEA HGLPLRLPQK DSPNQDATSW GPVRFLPMPG LLHEPQDQAG FLPPEPPDVG SSDPLSMVEP LQGRSPSYAS. It is sometimes possible for the material contained within the vial of "Fibroblast growth factor 21 (FGF-21), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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