Typical Testing Data/Standard Curve (for reference only)
Human Fibroblast growth factor 19 (FGF-19) ELISA Kit | FGF-19 elisa kit
Fibroblast growth factor 19 (FGF-19): Human (ELISA)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-19. Standards and samples are pipetted into the wells and any human FGF-19 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-19 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-19 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-19, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Fibroblast growth factor 19 (FGF-19) is a member of a subfamily of FGFs that includes FGF-21 and FGF-23, each member functions as an important regular of nutrient metabolism [1]. The primary source of endocrine FGF-19 is the ileum, bile acids release into the intestine after a meal to induce expression of FGF-19 [2]. Circulating FGF-19 plays an important role in maintaining proper bile acid homeostasis [3]. Several pharmacologic studies in hyperglycaemic, obese animal models have shown that FGF-19 can improve metabolic rate and lower serum glucose and hepatic triglyceride and cholesterol levels [4][5]. Like insulin, FGF-19 functions as postprandial hormone to govern hepatic protein synthesis, glycogen synthesis and gluconeogenesis, but does not stimulate lipogenesis [6].
2. Mini-review: endocrine actions of fibroblast growth factor 19. Jones S. Mol Pharm. 2008 Jan-Feb;5(1):42-8
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]
Uniprot Description
FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: extracellular region
Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; heart development; positive regulation of JNK cascade; positive regulation of glucose import; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of protein amino acid phosphorylation; neural crest cell migration
Research Articles on FGF-19
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Product Notes
The Human FGF-19 fgf19 (Catalog #AAA398052) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA398052 ELISA Kit recognizes Human FGF-19. It is sometimes possible for the material contained within the vial of "Fibroblast growth factor 19 (FGF-19), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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