Western Blot (WB)
(Western blot analysis of FGF21 in10ug non FGF21 expressing celllysate (Lane 1,3) and FGF21expressing cell lysate (Lane 2,4)using anti-FGF21 followed by goatanti-rabbit antibody. (The figure isfrom Prof. Cheah's lab, HKU.))
Rabbit anti-Mouse Fibroblast growth factor 21 (FGF-21) Polyclonal Antibody | anti-FGF-21 antibody
Fibroblast growth factor 21 (FGF-21): Mouse, Rabbit polyclonal (Antibody)
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Rabbit specific IgG was purified by mouse FGF-21 affinity chromatography
Synonyms
Fibroblast growth factor 21 (FGF-21); Polyclonal Antibody; Fibroblast growth factor 21 (FGF-21): Mouse; Rabbit polyclonal (Antibody); FGF-21; Polyclonal Antibody against Mouse FGF - 21; anti-FGF-21 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
The antibody detects circular mouse FGF-21.
Purity/Purification
Rabbit specific IgG was purified by mouse FGF-21 affinity chromatography
Form/Format
Solution in PBS
Sequence Length
209
Applicable Applications for anti-FGF-21 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: This antibody can be used at 2ug/mL with theappropriate secondary reagents to detect mouse FGF-21.
WB: This antibody can be used at0.2ug/mL~0.5ug/mL with the appropriate secondary reagentsto detect mouse FGF-21.
Immunoprecipitation and immunocytochemistry are not tested.
WB: This antibody can be used at0.2ug/mL~0.5ug/mL with the appropriate secondary reagentsto detect mouse FGF-21.
Immunoprecipitation and immunocytochemistry are not tested.
Immunogen
Recombinant full-length mouse FGF-21 expressed in E Coli
Preparation and Storage
Store at -20 degree C. For long-term storage, aliquot and freeze at -70 degree C. Avoid repeated freeze/defrost cycles.
Western Blot (WB)
(Western blot analysis of FGF21 in10ug non FGF21 expressing celllysate (Lane 1,3) and FGF21expressing cell lysate (Lane 2,4)using anti-FGF21 followed by goatanti-rabbit antibody. (The figure isfrom Prof. Cheah's lab, HKU.))
Western Blot (WB)
(Western blot analysis of FGF21 in10ug non FGF21 expressing celllysate (Lane 1,3) and FGF21expressing cell lysate (Lane 2,4)using anti-FGF21 followed by goatanti-rabbit antibody. (The figure isfrom Prof. Cheah's lab, HKU.))
Related Product Information for anti-FGF-21 antibody
Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic beta-cells. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from diet- induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance 7. Adiponectin has been shown to be critical in mediating the beneficial metabolic actions of FGF-21.
References
1. Stressed Liver and Muscle Call on Adipocytes with FGF21. Luo Y, McKeehan WL. Front Endocrinol 2013 18;4:194
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627- 1635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426- 437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203- 206
[4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687- 26695
[5] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[6] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96
[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627- 1635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426- 437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203- 206
[4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687- 26695
[5] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[6] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
Protein Family
Similar Products
Product Notes
The FGF-21 (Catalog #AAA398034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fibroblast growth factor 21 (FGF-21): Mouse, Rabbit polyclonal (Antibody) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Fibroblast growth factor 21 (FGF-21) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: This antibody can be used at 2ug/mL with theappropriate secondary reagents to detect mouse FGF-21. WB: This antibody can be used at0.2ug/mL~0.5ug/mL with the appropriate secondary reagentsto detect mouse FGF-21. Immunoprecipitation and immunocytochemistry are not tested. Researchers should empirically determine the suitability of the FGF-21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblast growth factor 21 (FGF-21), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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