Human Fibroblast Growth Factor 13 (FGF13) ELISA Kit | FGF13 elisa kit

Human Fibroblast Growth Factor 13 (FGF13) High Sensitive ELISA Kit

Cat. Num. AAA2087072

• Gene Names: FGF13; FGF2; FHF2; FHF-2; FGF-13
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 13 (FGF13); Human Fibroblast Growth Factor 13 (FGF13) High Sensitive ELISA Kit; FHF2; Fibroblast Growth Factor Homologous Factor 2; FGF13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibroblast Growth Factor 13 (FGF13).; No significant cross-reactivity or interference between High Sensitive Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
• Sequence Length: 199

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.23-100pg/mL
• Sensitivity: < 0.53pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF13 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 13 (FGF13) and unlabeled High Sensitive Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample.

Product Categories/Family for FGF13 elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 213417606
• NCBI GeneID: 2258
• NCBI Accession #: NP_001132970.1
• NCBI GenBank Nucleotide #: NM_001139498.1
• UniProt Accession #: Q92913; O95830; Q9NZH9; Q9NZI0; B1AK18; B7Z4M7; B7Z8N0; D3DWH4
• Molecular Weight: 25,607 Da
• NCBI Official Full Name: fibroblast growth factor 13 isoform 4
• NCBI Official Synonym Full Names: fibroblast growth factor 13
• NCBI Official Symbol: FGF13
• NCBI Official Synonym Symbols: FGF2; FHF2; FHF-2; FGF-13
• NCBI Protein Information: fibroblast growth factor 13
• UniProt Protein Name: Fibroblast growth factor 13
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF13
• UniProt Synonym Gene Names: FHF2; FGF-13; FHF-2

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq, Nov 2008]

Uniprot Description

Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus.