Rat Fibroblast Growth Factor 23 (FGF23) ELISA Kit | FGF23 elisa kit

Rat Fibroblast Growth Factor 23 (FGF23) ELISA Kit

Cat. Num. AAA2700282

• Gene Names: FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor 23 (FGF23); Rat Fibroblast Growth Factor 23 (FGF23) ELISA Kit; ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor; FGF23 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 23 (FGF23).; No significant cross-reactivity or interference between Fibroblast Growth Factor 23 (FGF23) and analogues was observed.
• Sequence Length: 251

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 2.47-200pg/mL
• Sensitivity: < 0.92pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 23 (FGF23) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 23 (FGF23) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF23 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 23 (FGF23) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 23 (FGF23) and unlabeled Fibroblast Growth Factor 23 (FGF23) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 23 (FGF23). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 23 (FGF23) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 23 (FGF23) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 10190674
• NCBI GeneID: 8074
• NCBI Accession #: NP_065689.1
• NCBI GenBank Nucleotide #: NM_020638.2
• UniProt Accession #: Q9GZV9; Q4V758
• Molecular Weight: 27,954 Da
• NCBI Official Full Name: fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: FGF23
• NCBI Official Synonym Symbols: ADHR; FGFN; HYPF; HPDR2; PHPTC
• NCBI Protein Information: fibroblast growth factor 23
• UniProt Protein Name: Fibroblast growth factor 23
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF23
• UniProt Synonym Gene Names: HYPF; FGF-23

NCBI Description

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]

Uniprot Description

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen

Molecular Function: 1-phosphatidylinositol-3-kinase activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; type 1 fibroblast growth factor receptor binding

Biological Process: cell differentiation; cellular phosphate ion homeostasis; cellular protein metabolic process; fibroblast growth factor receptor signaling pathway; MAPK cascade; negative regulation of bone mineralization; negative regulation of hormone secretion; negative regulation of osteoblast differentiation; peptidyl-tyrosine phosphorylation; phosphate ion homeostasis; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; positive regulation of GTPase activity; positive regulation of transcription, DNA-templated; post-translational protein modification; regulation of phosphatidylinositol 3-kinase signaling; response to magnesium ion; vitamin D catabolic process

Disease: Hypophosphatemic Rickets, Autosomal Dominant