Western Blot (WB)
(The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2S transfected 293T cell lysate)
Mouse anti-Human UBE2S Monoclonal Antibody | anti-UBE2S antibody
UBE2S antibody
Recommended starting dilution is 1:250
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2S transfected 293T cell lysate)
Western Blot (WB)
(The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2S transfected 293T cell lysate)
Immunofluorescence (IF)
(Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).)
Immunofluorescence (IF)
(Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).
Research Articles on UBE2S
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Product Notes
The UBE2S ube2s (Catalog #AAA200167) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UBE2S antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2S can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB/IF: 1:250 ~ 500. Recommended starting dilution is 1:250. Researchers should empirically determine the suitability of the UBE2S ube2s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2S, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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