Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit | ECE2 elisa kit

Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit

Cat. Num. AAA2024964

• Reactivity: Human
• Synonyms: Endothelin Converting Enzyme 2 (ECE2); Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit; Methyltransferase-like region; ECE2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Endothelin Converting Enzyme 2 (ECE2).; No significant cross-reactivity or interference between Endothelin Converting Enzyme 2 (ECE2) and analogues was observed.
• Sequence Length: 736

• Assay Type: Competitive Inhibition
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 123.46-10000pg/mL
• Sensitivity: < 45.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin Converting Enzyme 2 (ECE2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin Converting Enzyme 2 (ECE2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECE2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin Converting Enzyme 2 (ECE2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin Converting Enzyme 2 (ECE2) and unlabeled Endothelin Converting Enzyme 2 (ECE2) (Standards or samples) with the pre-coated antibody specific to Endothelin Converting Enzyme 2 (ECE2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin Converting Enzyme 2 (ECE2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin Converting Enzyme 2 (ECE2) in the sample.

Product Categories/Family for ECE2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 148745743
• NCBI GeneID: 9718
• Molecular Weight: 91,211 Da
• NCBI Official Full Name: Endothelin converting enzyme 2
• NCBI Official Synonym Full Names: endothelin converting enzyme 2
• NCBI Official Symbol: ECE2
• NCBI Protein Information: endothelin-converting enzyme 2
• UniProt Protein Name: Endothelin-converting enzyme 2
• Protein Family: Endothelin-converting enzyme
• UniProt Gene Name: ECE2
• UniProt Synonym Gene Names: KIAA0604; ECE-2
• UniProt Entry Name: ECE2_HUMAN

NCBI Description

This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ECE2: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.24.71; Methyltransferase, protein arginine, predicted; Vesicle; Methyltransferase; Protease

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: Golgi membrane; cytoplasmic vesicle membrane; integral to membrane

Molecular Function: methyltransferase activity; metalloendopeptidase activity; metal ion binding

Biological Process: methylation; cardioblast differentiation; cell-cell signaling; heart development; peptide hormone processing; brain development; proteolysis