Rabbit anti-Human, Mouse RIPK2 Polyclonal Antibody | anti-RIPK2 antibody

RIPK2 (Phospho-Ser176) Antibody

Cat. Num. AAA9408549

• Gene Names: RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: RIPK2; Polyclonal Antibody; RIPK2 (Phospho-Ser176) Antibody; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARDIAK; EC 2.7.11.1; kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP-2; RIP-like; anti-RIPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of RIPK2 only when phosphorylated at serine 176.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-RIPK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: peptide
• Immunogen Description: Peptide sequence around phosphorylation site of serine 176 (S-L-S(p)-Q-S) derived from Human RIPK2.
• Modification: Phospho-Ser176
• Target Name: RIPK2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with UV (15mins), using RIPK2 (Phospho-Ser176) antibody. The lane on the right is treated with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Phospho-Ser176) antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-RIPK2 antibody

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Product Categories/Family for anti-RIPK2 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506537
• NCBI GeneID: 8767
• NCBI Accession #: NP_003812.1
• NCBI GenBank Nucleotide #: NM_003821.5
• UniProt Accession #: O43353; Q6UWF0; B7Z748
• Molecular Weight: 45,582 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 2
• NCBI Official Symbol: RIPK2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK2
• UniProt Synonym Gene Names: CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
• UniProt Entry Name: RIPK2_HUMAN

NCBI Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; EC 2.7.10.2; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: protein complex; cytoskeleton; cytoplasm; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; protein binding; LIM domain binding; signal transducer activity; protein homodimerization activity; non-membrane spanning protein tyrosine kinase activity; CARD domain binding; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; positive regulation of interleukin-12 production; positive regulation of apoptosis; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; signal transduction; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; T cell proliferation; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; positive regulation of immature T cell proliferation; positive regulation of interferon-beta production; lipopolysaccharide-mediated signaling pathway; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of protein ubiquitination; toll-like receptor signaling pathway; positive regulation of interleukin-2 production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation; toll-like receptor 9 signaling pathway; positive regulation of T-helper 1 cell differentiation; positive regulation of cytokine and chemokine mediated signaling pathway; negative regulation of apoptosis