Rabbit anti-Human MMP20 Polyclonal Antibody | anti-MMP20 antibody

MMP20, CT (MMP20, Matrix metalloproteinase-20, Enamel metalloproteinase, Enamelysin) (PE)

Cat. Num. AAA6321426

• Gene Names: MMP20; AI2A2; MMP-20
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MMP20; Polyclonal Antibody; CT (MMP20; Matrix metalloproteinase-20; Enamel metalloproteinase; Enamelysin) (PE); Enamelysin; anti-MMP20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP20.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MMP20 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 399-428 in the C-terminal region of human MMP20, UniProt Accession #NP_004762.2;O60882. Species sequence homology: bovine
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane)MBS6006022. This demonstrates that MBS6006022 detected the MMP20 protein (arrow).)

Related Product Information for anti-MMP20 antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and so the protein is thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes that localizes to chromosome 11q22.3.

Product Categories/Family for anti-MMP20 antibody

Antibodies; Matrix Metalloproteinases

References

1. Bailey, S.D., et al. Diabetes Care (2010) In press 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Wojciechowski, R., et al. Invest. Ophthalmol. Vis. Sci. (2010) In press 4. Lee, S.K., et al. J. Dent. Res. 89(1):46-50(2010). 5. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 45359865
• NCBI GeneID: 9313
• NCBI Accession #: NP_004762.2
• NCBI GenBank Nucleotide #: NM_004771.3
• UniProt Accession #: O60882; Q6DKT9
• Molecular Weight: 54,387 Da
• NCBI Official Full Name: matrix metalloproteinase-20 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 20
• NCBI Official Symbol: MMP20
• NCBI Official Synonym Symbols: AI2A2; MMP-20
• NCBI Protein Information: matrix metalloproteinase-20
• UniProt Protein Name: Matrix metalloproteinase-20
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP20
• UniProt Synonym Gene Names: MMP-20

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]

Uniprot Description

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.