ECE2 recombinant protein

ECE2 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120100

• Purity: >95% as determined by SDS-PAGE
• Synonyms: ECE2; ECE2 Protein; Human; Recombinant (hFc Tag); Human ECE-2 Protein (Fc Tag); KIAA0604 Protein; UNQ403/PRO740 Protein; endothelin converting enzyme 2; ECE2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly199-Trp883

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the ectodomain of human endothelin converting enzyme 2 isoform A (NP_055508.3)(Gly 199-Trp 883) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ECE2 recombinant protein

Background: Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.

References

Lorenzo M.-N., et al.,(2001), Human endothelin converting enzyme-2 (ECE2): characterization of mRNA species and chromosomal localization. Biochim. Biophys. Acta 1522:46-52.Muzny D.M., et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.Nagase T., et al.,(1998), Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.DNA Res. 5:31-39.

NCBI and Uniprot Product Information

• NCBI GI #: 153945761
• NCBI GeneID: 9718
• NCBI Accession #: NP_055508.3
• NCBI GenBank Nucleotide #: NM_014693.3
• UniProt Accession #: O60344; Q6NTG7; Q6UW36; Q8NFD7; Q96NX3; Q96NX4; Q9BRZ8; A5PLK8
• Molecular Weight: 91,211 Da
• NCBI Official Full Name: endothelin-converting enzyme 2 isoform A
• NCBI Official Synonym Full Names: endothelin converting enzyme 2
• NCBI Official Symbol: ECE2
• NCBI Protein Information: endothelin-converting enzyme 2
• UniProt Protein Name: Endothelin-converting enzyme 2
• Protein Family: Endothelin-converting enzyme
• UniProt Gene Name: ECE2
• UniProt Synonym Gene Names: KIAA0604; UNQ403/PRO740; ECE-2
• UniProt Entry Name: ECE2_HUMAN

NCBI Description

This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]