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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD300a (MEM-260) APC.)

Mouse anti-Human CD300a Antibody | anti-CD300a antibody

Anti-Hu CD300a APC

Gene Names
CD300A; IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD300a; Anti-Hu CD300a APC; CD300a molecule; IRC; CLM-8; IGSF12; CMRF35H; anti-CD300a antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
MEM-260
Specificity
The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
186
Applicable Applications for anti-CD300a antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
HPB human acute lymphoid leukemia cell line
Conjugation
APC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD300a (MEM-260) APC.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD300a (MEM-260) APC.)
Related Product Information for anti-CD300a antibody
CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CMRF35-like molecule 8 isoform 2
NCBI Official Synonym Full Names
CD300a molecule
NCBI Official Symbol
CD300A
NCBI Official Synonym Symbols
IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
NCBI Protein Information
CMRF35-like molecule 8
UniProt Protein Name
CMRF35-like molecule 8
Protein Family
UniProt Gene Name
CD300A
UniProt Synonym Gene Names
CMRF35H; IGSF12; CLM-8; CMRF35-H9; IgSF12; IRp60
UniProt Entry Name
CLM8_HUMAN

NCBI Description

This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MAIR-I: Inhibitory receptor which may contribute to the down- regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Belongs to the CD300 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylserine binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of MAP kinase activity; negative regulation of NK T cell activation; immune system process; negative regulation of mast cell degranulation; negative regulation of mast cell activation during immune response; negative regulation of B cell proliferation; signal transduction; negative regulation of phagocytosis, engulfment; negative regulation of fibroblast proliferation; negative regulation of B cell receptor signaling pathway; regulation of T cell receptor signaling pathway; cell adhesion

Research Articles on CD300a

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Product Notes

The CD300a cd300a (Catalog #AAA1801175) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD300a APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD300a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD300a cd300a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD300a, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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