Human Diacylglycerol Kinase Theta (DGKQ) ELISA Kit | DGKQ elisa kit

Human Diacylglycerol Kinase Theta (DGKQ) ELISA Kit

Cat. Num. AAA9715740

• Gene Names: DGKQ; DAGK; DAGK4; DAGK7
• Reactivity: Human
• Synonyms: Diacylglycerol Kinase Theta (DGKQ); Human Diacylglycerol Kinase Theta (DGKQ) ELISA Kit; DGKQ; DAGK; DAGK4; DAGK7; diacylglycerol kinase; theta; DGKQ elisa kit

• Reactivity: Human
• Specificity: Human Diacylglycerol kinase theta (DGKQ) ELISA Kit has high sensitivity and excellent specificity for detection of Human DGKQ. No significant cross-reactivity or interference between Human DGKQ and analogues was observed.
• Sequence Length: 942

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Diacylglycerol kinase theta (DGKQ) Standard Curve.)

Related Product Information for DGKQ elisa kit

Description: This Human Diacylglycerol kinase theta (DGKQ) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKQ in samples. An antibody specific for DGKQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DGKQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The protein encoded by DGKQ contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction.

NCBI and Uniprot Product Information

• NCBI GI #: 40806175
• NCBI GeneID: 1609
• NCBI Accession #: NP_001338.2
• NCBI GenBank Nucleotide #: NP_001338.2
• UniProt Accession #: P52824
• NCBI Official Full Name: diacylglycerol kinase theta
• NCBI Official Synonym Full Names: diacylglycerol kinase theta
• NCBI Official Symbol: DGKQ
• NCBI Official Synonym Symbols: DAGK; DAGK4; DAGK7
• NCBI Protein Information: diacylglycerol kinase theta
• UniProt Protein Name: Diacylglycerol kinase theta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKQ
• UniProt Synonym Gene Names: DAGK4; DAG kinase theta; DGK-theta
• UniProt Entry Name: DGKQ_HUMAN

NCBI Description

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP