Goat DGKQ Polyclonal Antibody | anti-DGKQ antibody

Goat Polyclonal to Human DGKQ

Cat. Num. AAA248944

• Gene Names: DGKQ; DAGK; DAGK4; DAGK7
• Reactivity: Human, Orangutan
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DGKQ; Polyclonal Antibody; Goat Polyclonal to Human DGKQ; Anti-DGKQ Antibody (aa295-298) IHC-plus; DAGK7; DAG kinase theta; DAGK4; Diglyceride kinase theta; DAGK; DGK-theta; Diacylglycerol kinase theta; Human DGKQ; anti-DGKQ antibody

• Host: Goat
• Reactivity: Human, Orangutan
• Clonality: Polyclonal
• Specificity: Human DGKQ.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 942

• Applicable Applications for anti-DGKQ antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments in Human Brain lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
• Target Species: Human
• Immunogen Description: Synthetic peptide C-PGRETQATPESGKQ from an internal region of human DGKQ (NP_001338.2). Percent identity by BLAST analysis: Human, Orangutan (100%); Gibbon (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: DGKQ antibody was raised against synthetic peptide C-PGRETQATPESGKQ from an internal region of human DGKQ (NP_001338.2). Percent identity by BLAST analysis: Human, Orangutan (100%); Gibbon (93%).
• Antigen Modification: aa295-298
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-DGKQ antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-DGKQ antibody

Diacylglycerol kinase, theta (110kD) mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction.

Product Categories/Family for anti-DGKQ antibody

Family: Non-Protein Kinase; Subfamily: Diacylglycerol kinase

NCBI and Uniprot Product Information

• NCBI GI #: 40806175
• NCBI GeneID: 1609
• NCBI Accession #: NP_001338.2
• NCBI GenBank Nucleotide #: NM_001347.3
• UniProt Accession #: P52824; Q6P3W4
• Molecular Weight: 101,155 Da
• NCBI Official Full Name: diacylglycerol kinase theta
• NCBI Official Synonym Full Names: diacylglycerol kinase, theta 110kDa
• NCBI Official Symbol: DGKQ
• NCBI Official Synonym Symbols: DAGK; DAGK4; DAGK7
• NCBI Protein Information: diacylglycerol kinase theta; DGK-theta; DAG kinase theta; diglyceride kinase theta
• UniProt Protein Name: Diacylglycerol kinase theta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKQ
• UniProt Synonym Gene Names: DAGK4; DAG kinase theta; DGK-theta
• UniProt Entry Name: DGKQ_HUMAN

NCBI Description

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Lipid Metabolism - glycerolipid; Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP