Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) Recombinant Protein | MYH3 recombinant protein

Recombinant Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3)

Cat. Num. AAA2009871

• Gene Names: Myh3; Myhse; RNMHCG
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myosin Heavy Chain 3; Skeletal Muscle; Embryonic (MYH3); Recombinant Myosin Heavy Chain 3; MYH3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RTVETMQG ALDAEVRSRN EAIRLKKKME GDLNEIEIQL SHANRQAAET IKHLRSVQGQ LKDTQLHLDD ALRGQEDLKE QLAIVERRAN LLQAEVEELR ATLEQTERAR KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLTQLQS EVEDASRDAR NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE AEQLALKGGK KQIQKLETRI RELEFELEGE QKRNTESVKG LRKYERRV
• Sequence Length: 1940

• Applicable Applications for MYH3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg1593~Val1848 (Accession # P12847) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6981234
• NCBI GeneID: 24583
• NCBI Accession #: NP_036736.1
• NCBI GenBank Nucleotide #: NM_012604.1
• UniProt Accession #: P12847
• Molecular Weight: 33.5kDa
• NCBI Official Full Name: myosin-3
• NCBI Official Synonym Full Names: myosin, heavy chain 3, skeletal muscle, embryonic
• NCBI Official Symbol: Myh3
• NCBI Official Synonym Symbols: Myhse; RNMHCG
• NCBI Protein Information: myosin-3; myosin heavy chain 3; myosin heavy polypeptide 3; myosin heavy chain, fast skeletal muscle, embryonic; myosin, heavy polypeptide 3, skeletal muscle, embryonic
• UniProt Protein Name: Myosin-3
• UniProt Gene Name: Myh3
• UniProt Entry Name: MYH3_RAT

NCBI Description

heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

Uniprot Description

MYH3: Muscle contraction. Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A); also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant. Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B); also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.

Protein type: Motility/polarity/chemotaxis; Motor

Cellular Component: myofibril; contractile fiber; myosin complex

Molecular Function: microfilament motor activity; calmodulin binding; actin filament binding; motor activity; actin binding; ATP binding

Biological Process: skeletal muscle contraction; metabolic process