Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Diacylglycerol kinase theta (DGKQ) Standard Curve.)
Mouse Diacylglycerol Kinase Theta (DGKQ) ELISA Kit | DGKQ elisa kit
Mouse Diacylglycerol Kinase Theta (DGKQ) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Diacylglycerol kinase theta (DGKQ) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Diacylglycerol kinase theta (DGKQ) Standard Curve.)
Background: The protein encoded by DGKQ contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction.
NCBI and Uniprot Product Information
Uniprot Description
DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.
Protein type: Lipid Metabolism - glycerophospholipid; Kinase, lipid; Lipid Metabolism - glycerolipid; EC 2.7.1.107; Motility/polarity/chemotaxis
Cellular Component: cytoskeleton; membrane; cytoplasm; plasma membrane; nuclear speck; nucleus; cytosol
Molecular Function: transferase activity; phospholipase binding; transcription activator binding; metal ion binding; nucleotide binding; kinase activity; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: regulation of transcription from RNA polymerase II promoter; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; metabolic process; protein kinase C activation; signal transduction; phosphorylation; response to ATP
Research Articles on DGKQ
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Product Notes
The Mouse DGKQ dgkq (Catalog #AAA9717907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717907 ELISA Kit recognizes Mouse DGKQ. It is sometimes possible for the material contained within the vial of "Diacylglycerol Kinase Theta (DGKQ), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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