Testing Data
Mouse GAD65 Monoclonal Antibody | anti-GAD65 antibody
Mouse Anti-GAD65 [144]
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, immunoassay, Radioimmunoassay
Purity
Affinity purified
Synonyms
GAD65; Monoclonal Antibody; Mouse Anti-GAD65 [144]; 65 kDa glutamic acid decarboxylase antibody; DCE 2 antibody; DCE2 antibody; GAD 2 antibody; GAD 65antibody; GAD-2 antibody; GAD-65 antibody; GAD2 antibody; Glutamate Decarboxylase 2 (pancreatic islets and brain65kDa) antibody; Glutamate Decarboxylase 2 antibody; Glutamate Decarboxylase 65 antibody; Glutamate decarboxylase65 kDa isoform antibody; Glutamic Acid Decarboxylase 2 antibody; Glutamic Acid Decarboxylase 65antibody; anti-GAD65 antibody
Host
Mouse
Reactivity
Mouse, Rat, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
144
Purity/Purification
Affinity purified
Form/Format
Purified antibody containing PBS 0.1% sodium azide;
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GAD65 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Radioimmunoassay (RIA)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try WB: Use a concentration of 1 ug/ml. Predicted molecular weight: 65 kDa
Target Antigen
GAD65
Immunogen
Synthetic peptide: GSGFWSFGSEDGSGDSEN conjugated to KLH, corresponding to amino acids 4-22 of Human GAD65
Myeloma/fusion Partners
Sp2/0 Ag.14
Testing Data
Testing Data
References
Antigen presentation of detergentfree glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein. Steed J et al (J of Immunol Methods,2008) PUBMED Singh S et al. Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment. BMC Mol Biol 13:28 (2012). WB PUBMED Hampe CS et al. A novel monoclonal antibody specific for the N-terminal end of GAD65. J Neuroimmunol 113:63-71 (2001) PUBMED
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Product Notes
The GAD65 (Catalog #AAA375043) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-GAD65 [144] reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAD65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Radioimmunoassay (RIA). Optimal antibody dilution should be determined by titration, however as a guideline try WB: Use a concentration of 1 ug/ml. Predicted molecular weight: 65 kDa. Researchers should empirically determine the suitability of the GAD65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAD65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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