Human Dengue Virus IgM ELISA Kit
Human Dengue Virus IgM ELISA Kit
Reactivity
Human
Synonyms
Dengue Virus IgM; Human Dengue Virus IgM ELISA Kit; Dengue Virus IgM elisa kit
Reactivity
Human
Specificity
The Dengue virus IgG ELISA and IgM ELISA was verified in an internal study. The sensitivity and specificity in both cases exceeded 96%.
Samples
Serum Or Plasma Directed Against Dengue Virus (Den 1-4).
Preparation and Storage
Storage 2 - 8 degree C
Related Product Information for Dengue Virus IgM elisa kit
Intended Uses: The Dengue Virus IgG and IgM ELISA tests are qualitative immunoassays for the detection of human antibodies in serum or plasma directed against Dengue virus (DEN 1-4).
Principle of the Assay: The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: While the Dengue Virus IgM ELISA test utilizes inactivated virus lysat, recombinantly expressed peptides from the envelope protein are the fundamental component for antibody determination with the Dengue Virus IgG ELISA. In mature virus particles, dimers of the E-proteins on the viral surface initiate the contact to the host cells. The Envelope (E) protein monomer can be divided into three different domains, named DI, DII and DIII. Whilst DI and DIII contain predominantely subcomplex-and type-specific epitopes, DII presents the major flavivirus group and subgroup cross reactive epitopes. The usage of subcomplex and type-specific components of the envelope proteins as antigen for antibody detection reduces cross-reactivity which improves the validity of results, particularly of the Dengue Virus IgG ELISA test.
Principle of the Assay: The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: While the Dengue Virus IgM ELISA test utilizes inactivated virus lysat, recombinantly expressed peptides from the envelope protein are the fundamental component for antibody determination with the Dengue Virus IgG ELISA. In mature virus particles, dimers of the E-proteins on the viral surface initiate the contact to the host cells. The Envelope (E) protein monomer can be divided into three different domains, named DI, DII and DIII. Whilst DI and DIII contain predominantely subcomplex-and type-specific epitopes, DII presents the major flavivirus group and subgroup cross reactive epitopes. The usage of subcomplex and type-specific components of the envelope proteins as antigen for antibody detection reduces cross-reactivity which improves the validity of results, particularly of the Dengue Virus IgG ELISA test.
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Product Notes
The Human Dengue Virus IgM (Catalog #AAA495198) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495198 ELISA Kit recognizes Human Dengue Virus IgM. It is sometimes possible for the material contained within the vial of "Dengue Virus IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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