Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) RTU ELISA Kit | PTGS2 rtu elisa kit

Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit

Cat. Num. AAA4502097

• Gene Names: Ptgs2; Cox2; COX-2
• Reactivity: Rat
• Synonyms: Prostaglandin Endoperoxide Synthase 2 (PTGS2); Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit; PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 604

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 25.7pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGS2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTGS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PTGS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTGS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTGS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148747270
• NCBI GeneID: 29527
• NCBI Accession #: NP_058928.3
• NCBI GenBank Nucleotide #: NM_017232.3
• UniProt Accession #: P35355; Q64379; Q925V4
• Molecular Weight: 69,164 Da
• NCBI Official Full Name: prostaglandin G/H synthase 2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2
• NCBI Official Symbol: Ptgs2
• NCBI Official Synonym Symbols: Cox2; COX-2
• NCBI Protein Information: prostaglandin G/H synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: Ptgs2
• UniProt Synonym Gene Names: Cox-2; Cox2; COX-2; PGH synthase 2; PGHS-2

NCBI Description

catalyzes the conversion of arachidonic acid products to prostaglandin; may play a role in response to endotoxic shock; may a role in long term potentiation [RGD, Feb 2006]

Uniprot Description

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.