Mouse Leptin ELISA Kit | LEP elisa kit

Mouse Leptin ELISA Kit

Cat. Num. AAA560911

• Gene Names: LEP; OB; OBS; LEPD
• Reactivity: Mouse
• Synonyms: Leptin; Mouse Leptin ELISA Kit; LEP elisa kit

• Reactivity: Mouse
• Specificity: The Leishmania IgG ELISA was verified in an internal study. The sensitivity and specificity in both cases exceeded 99%.
• Sequence Length: 167

• Samples: Various Kinds Of Samples.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LEP elisa kit

Intended Uses: The Leishmania IgG ELISA test is a qualitative immunoassay for the demonstration of IgG antibodies in serum or plasma directed against Leishmania. The assay is recommended for the sensitive detection of such antibodies in various kinds of samples.

Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Leishmaniasis is an infectious disease, caused by protozoic parasites of the genus Leishmania. The disease occurs predominantly in tropical and subtropical climate zones. Primarily affected are farm and domestic animals, however the disease can also be transmitted to humans. Leishmaniasis is distributed worldwide with high incidences in Eastern Africa, Southern America and Asia. In Europe, the Mediterranean area is particularly affected. Annually, some 1.5 million cases of cutaneous and another 0.5 million cases of visceral leishmaniases are reported. Around 12 million people are infected with Leishmania worldwide. Dogs and rodents serve as the main reservoirs for Leishmania, but also cats, horses, sheep and cattle may be afflicted. Sandflies (phlebotominae) or other moth flies (psychodidae) transmit the parasites to humans. The incubation period is very variable, ranging from a few weeks up to several years. ELISA test systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.3
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• Protein Family: Leptin

NCBI Description

This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]