Human CXCL12/SDF-1 ELISA Kit | CXCL12 elisa kit

Human CXCL12/SDF-1 ELISA Kit

Cat. Num. AAA9135919

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• Reactivity: Human
• Synonyms: CXCL12/SDF-1; Human CXCL12/SDF-1 ELISA Kit; CXCL12 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes both recombinant and natural human CXCL12/SDF-1. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
• Sequence Length: 93

• Assay Type: Quantitative Sandwich
• Sensitivity: < 10.94pg/mL
• Intra-assay Precision: 3 samples with low, middle and high level CXCL12/SDF-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: 3 samples with low, middle and high level CXCL12/SDF-1 were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<15%
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL12 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human CXCL12/SDF-1 has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any CXCL12/SDF-1 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human CXCL12/SDF-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL12/SDF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CXCL12/SDF-1 in human serum, plasma, cell culture supernates and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 10834988
• NCBI GeneID: 6387
• NCBI Accession #: NP_000600.1
• NCBI GenBank Nucleotide #: NM_000609.6
• UniProt Accession #: P48061; Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
• Molecular Weight: 10,103 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1
• UniProt Protein Name: Stromal cell-derived factor 1
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: CXCL12
• UniProt Synonym Gene Names: SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF

NCBI Description

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells ().