Human stromal cell derived factor 1beta ELISA Kit | SDF-1beta/SDF1B elisa kit

Human stromal cell derived factor 1beta, SDF-1beta/SDF1B ELISA Kit

Cat. Num. AAA700136

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• Reactivity: Human
• Synonyms: stromal cell derived factor 1beta; Human stromal cell derived factor 1beta; SDF-1beta/SDF1B ELISA Kit; Human stromal cell derived factor 1beta (SDF-1beta/SDF1B) ELISA kit; SDF-1beta/SDF1B elisa kit

• Reactivity: Human
• Sequence Length: 93

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 125 pg/ml-8000 pg/ml
• Sensitivity: 31.25 pg/ml
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 2: chemokine
• Protein Biological Process 3: Chemotaxis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDF-1beta/SDF1B elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SDF-1beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SDF-1beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SDF-1beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10834988
• NCBI GeneID: 6387
• NCBI Accession #: NP_000600.1
• NCBI GenBank Nucleotide #: NM_000609.6
• UniProt Accession #: P48061; Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
• Molecular Weight: 10,666 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
• UniProt Protein Name: Stromal cell-derived factor 1
• UniProt Gene Name: CXCL12
• UniProt Synonym Gene Names: SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
• UniProt Entry Name: SDF1_HUMAN

NCBI Description

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.6 PublicationsManual assertion based on experiment in:Ref.14