Rabbit anti-Human, Mouse c-Abl Monoclonal Antibody

c-Abl, phosphorylated (Tyr204) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

Cat. Num. AAA605600

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: c-Abl; Monoclonal Antibody; phosphorylated (Tyr204) (ABL1; v-abl; Abelson Murine Leukemia Viral Oncogene Homolog 1; ABL; c-ABL; JTK7; p150; Proto-Oncogene Tyrosine-Protein Kanase ABL1; v-abl); Anti -c-Abl; anti-c-Abl antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10g163
• Specificity: Recognizes endogenous levels of c-Abl only when phosphorylated at Tyr204. Species Sequence Homology: Mouse.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-c-Abl antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr204 of human c-Abl.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from CML-T1 leukemic cells using MBS605600 (upper and middle) or c-Abl Antibody (lower). The phospho-specificity of this rabbit mAb was verified by treating the membrane with calf intestinal phosphatase (CIP) (middle and lower) before antibody probing.)

Related Product Information for anti-c-Abl antibody

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

Product Categories/Family for anti-c-Abl antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 179750
• NCBI Official Full Name: c-abl