anti-Human STAT1 Polyclonal Antibody | anti-STAT1 antibody

Anti-STAT1 Rabbit pAb

Cat. Num. AAA477126

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: STAT1; Polyclonal Antibody; Anti-STAT1 Rabbit pAb; Signal transducer and activator of transcription 1 91kD; DKFZp686B04100; ISGF 3; ISGF-3; OTTHUMP00000163552; OTTHUMP00000165046; OTTHUMP00000165047; OTTHUMP00000205845; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1 alpha/beta; Signal transducer and activator of transcription 1; 91kD; Signal transducer and activator of transcription 1-alpha/beta; Signal Transductor and Activator of Transcription 1; STAT 1; STAT 91; Stat1; STAT1_HUMAN; STAT91; Transcription factor ISGF 3 components p91 p84; Transcription factor ISGF-3 components p91/p84; anti-STAT1 antibody

• Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: 10mM PBS with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 750

• Applicable Applications for anti-STAT1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727.
• Sensitivity: STAT1 (Ab-727) antibody detects endogenous levels of total STAT1 protein.
• Sequence Similarities: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
• Subunit Structure: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter. Interacts with HCV core protein; the interaction results in STAT1 degradation. Interacts with PIAS1; the interaction requires phosphorylation on Ser-727 and inhibits STAT1 activation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with PIAS1 (dimethylated on arginine); the interaction results in release of STAT1 from its target gene. Interacts with SRC. Interacts with ERBB4 (phosphorylated). Interacts with PTK2/FAK1.
• Subcellular Location: Cytoplasm. Nucleus. Note: Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using STAT1 (Ab-727) antibody.)

Related Product Information for anti-STAT1 antibody

Entrez Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 6274552
• NCBI GeneID: 6772
• NCBI Accession #: NP_009330.1
• NCBI GenBank Nucleotide #: NM_007315
• UniProt Accession #: P42224; Q53S88; Q53XW4; Q68D00; Q9UDL5; A8K989; B2RCA0; D2KFR8; D3DPI7
• Molecular Weight: 87
• NCBI Official Full Name: signal transducer and activator of transcription 1-alpha/beta isoform alpha
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT1

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: axon; cell-cell adherens junction; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: double-stranded DNA binding; enzyme binding; identical protein binding; nuclear hormone receptor binding; protein binding; protein homodimerization activity; transcription factor activity; tumor necrosis factor receptor binding

Biological Process: apoptosis; blood circulation; endothelial cell migration; JAK-STAT cascade; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; response to cAMP; response to cytokine stimulus; response to peptide hormone stimulus; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c