Mouse anti-Human CD86 Monoclonal Antibody | anti-CD86 antibody

Anti-CD86 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8103599

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD86; Monoclonal Antibody; Anti-CD86 Antibody (PE); Mouse Monoclonal; CD86/B7-2 Antibody (PE); Mouse MAb; CD86 antigen; Anti-B7-2 Antibody; Anti-B7.2 Antibody; Anti-B70 Antibody; Anti-CD28LG2 Antibody; Anti-LAB72 Antibody; anti-CD86 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 06
• Specificity: Human CD86/B7-2
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD86 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human CD86/B7-2 protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD86/B7-2 (rh CD86/B7-2) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-B7-2 (CD86) reactivity on Daudi cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with 20 ug of human IgG/106 cells for 1 hour at 4 degree C prior to staining, washed, then stained with PE Mouse anti-B7-2 (CD86).)

Related Product Information for anti-CD86 antibody

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Product Categories/Family for anti-CD86 antibody

Cancer Drug Targets

References

Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.

NCBI and Uniprot Product Information

• NCBI GI #: 91208433
• NCBI GeneID: 942
• NCBI Accession #: NP_008820.2
• NCBI GenBank Nucleotide #: NM_006889.3
• Molecular Weight: 28,420 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86 isoform 2
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]