Human CHIT1 ELISA Kit | CHIT1 elisa kit

Human CHIT1 PicoKine ELISA Kit

Cat. Num. AAA1752637

• Gene Names: CHIT1; CHI3; CHIT; CHITD
• Reactivity: Human
• Synonyms: CHIT1; Human CHIT1 PicoKine ELISA Kit; Human CHIT1; chitinase 1; Chitotriosidase-1; Chitinase-1; CHIT1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human 1118
• Sequence Length: 466

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHIT1 elisa kit

Principle of the Assay: The Picokine™ Human 1118 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human 1118 with a 96-well strip plate that is pre-coated with antibody specific for 1118. The detection antibody is a biotinylated antibody specific for 1118. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human 1118 with immunogen: Expression system for standard: NSO, Immunogen sequence: A22-N466. The kit is analytically validated with ready to use reagents. To measure Human 1118, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human 1118 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human 1118 in the sample.!!Background/Introduction: Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 37999493
• NCBI GeneID: 1118
• UniProt Accession #: Q13231
• NCBI Official Full Name: Chitotriosidase-1
• NCBI Official Synonym Full Names: chitinase 1
• NCBI Official Symbol: CHIT1
• NCBI Official Synonym Symbols: CHI3; CHIT; CHITD
• NCBI Protein Information: chitotriosidase-1
• UniProt Protein Name: Chitotriosidase-1
• Protein Family: Chitotriosidase
• UniProt Gene Name: CHIT1
• UniProt Entry Name: CHIT1_HUMAN

NCBI Description

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; lysosome

Molecular Function: endochitinase activity; chitin binding; chitinase activity

Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process

Disease: Chitotriosidase Deficiency