Human GAS1 ELISA Kit | GAS1 elisa kit

Human GAS1 PicoKine ELISA Kit

Cat. Num. AAA1752649

• Reactivity: Human
• Synonyms: GAS1; Human GAS1 PicoKine ELISA Kit; Human GAS1; growth arrest specific 1; Growth arrest-specific protein 1; GAS-1; GAS1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human GAS1
• Sequence Length: 345

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAS1 elisa kit

Principle of the Assay: The Picokine™ Human GAS1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human GAS1 with a 96-well strip plate that is pre-coated with antibody specific for GAS1. The detection antibody is a biotinylated antibody specific for GAS1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human GAS1 with immunogen: Expression system for standard: NSO, Immunogen sequence: L40-S318. The kit is analytically validated with ready to use reagents. To measure Human GAS1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human GAS1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human GAS1 in the sample.!!Background/Introduction: Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 9q21.33. Growth arrestspecific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

NCBI and Uniprot Product Information

• NCBI GI #: 167466169
• NCBI GeneID: 2619
• NCBI Accession #: NP_002039.2
• NCBI GenBank Nucleotide #: NP_002039.2
• UniProt Accession #: P54826
• NCBI Official Full Name: growth arrest-specific protein 1
• NCBI Official Synonym Full Names: growth arrest specific 1
• NCBI Official Symbol: GAS1
• NCBI Protein Information: growth arrest-specific protein 1
• UniProt Protein Name: Growth arrest-specific protein 1
• Protein Family: Germacrene A synthase
• UniProt Gene Name: GAS1
• UniProt Synonym Gene Names: GAS-1
• UniProt Entry Name: GAS1_HUMAN

NCBI Description

Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAS1: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 9q21.3-q22

Cellular Component: anchored to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: developmental growth; axon guidance; cell fate commitment; camera-type eye development; outer ear morphogenesis; programmed cell death; cerebellum morphogenesis; palate development; embryonic cranial skeleton morphogenesis; middle ear morphogenesis; odontogenesis; regulation of apoptosis; negative regulation of smoothened signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; negative regulation of mitotic cell cycle; embryonic digit morphogenesis; negative regulation of cell growth; cell cycle arrest; positive regulation of epithelial cell proliferation; dorsoventral neural tube patterning; regulation of smoothened signaling pathway; negative regulation of epithelial cell proliferation; eye morphogenesis