Typical Testing Data/Standard Curve (for reference only)
Mouse CHIT1 ELISA Kit | CHIT1 elisa kit
Mouse CHIT1 PicoKine ELISA Kit
Gene Names
Chit1; 2300002L19Rik
Reactivity
Mouse
Synonyms
CHIT1; Mouse CHIT1 PicoKine ELISA Kit; Mouse CHIT1; chitinase 1 (chitotriosidase); Chitotriosidase-1; Chitinase-1; Chit1; CHIT1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Chit1
Sequence Length
396
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312pg/ml-20000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CHIT1 elisa kit
Principle of the Assay: The Boster Picokine™ Mouse Chit1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Chit1 with a 96-well strip plate that is pre-coated with antibody specific for Chit1. The detection antibody is a biotinylated antibody specific for Chit1. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Chit1 with immunogen: Expression system for standard: NSO, Immunogen sequence: A22-S464. The kit is analytically validated with ready to use reagents. To measure Mouse Chit1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Chit1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Chit1 in the sample.!!Background/Introduction: Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1; 1 E4. It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract. CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form. Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease. The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme. Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
chitotriosidase-1 isoform 2
NCBI Official Synonym Full Names
chitinase 1 (chitotriosidase)
NCBI Official Symbol
Chit1
NCBI Official Synonym Symbols
2300002L19Rik
NCBI Protein Information
chitotriosidase-1
UniProt Protein Name
Chitotriosidase-1
Protein Family
UniProt Gene Name
Chit1
Uniprot Description
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens ().
Research Articles on CHIT1
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Product Notes
The Mouse CHIT1 chit1 (Catalog #AAA1752638) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752638 ELISA Kit recognizes Mouse CHIT1. It is sometimes possible for the material contained within the vial of "CHIT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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