Typical Testing Data/Standard Curve (for reference only)
Human BMP-7 ELISA Kit | BMP-7 elisa kit
BMP-7 Human ELISA Kit
Gene Names
bmp7.2; bmp7
Reactivity
Human
Applications
ELISA
Synonyms
BMP-7; BMP-7 Human ELISA Kit; Human BMP-7; BMP-7 elisa kit
Reactivity
Human
Applicable Applications for BMP-7 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of BMP-7 in human serum, plasma, urine, bone tissue or cell culture supernatants.
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 10pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BMP-7 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-7 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-7 can be calculated.
Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a member of the TGF-beta superfamily. It is expressed in the brain, kidneys and bladder. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a member of the TGF-beta superfamily. It is expressed in the brain, kidneys and bladder. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,965 Da
NCBI Official Full Name
bone morphogenetic protein 7
NCBI Official Synonym Full Names
bone morphogenetic protein 7, gene 2<
NCBI Official Symbol
bmp7.2
NCBI Official Synonym Symbols
bmp7
NCBI Protein Information
bone morphogenetic protein 7; OP-1; BMP-7; xBMP7; osteogenic protein 1
UniProt Protein Name
Bone morphogenetic protein 7
UniProt Gene Name
bmp7
UniProt Synonym Gene Names
BMP-7; xBMP7; OP-1
UniProt Entry Name
BMP7_XENLA
Uniprot Description
Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Subunit structure: Homodimer; disulfide-linked. Interacts with twsg1. Ref.3
Subcellular location: Secreted
By similarity.
Sequence similarities: Belongs to the TGF-beta family.
Research Articles on BMP-7
Similar Products
Product Notes
The Human BMP-7 bmp7 (Catalog #AAA355414) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355414 ELISA Kit recognizes Human BMP-7. AAA Biotech's BMP-7 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of BMP-7 in human serum, plasma, urine, bone tissue or cell culture supernatants. Researchers should empirically determine the suitability of the BMP-7 bmp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP-7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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