Frizzled-1 / FZD1 Recombinant Protein | FZD1 recombinant protein

Recombinant Mouse Frizzled-1 / FZD1 Protein (His tag)

Cat. Num. AAA2547167

• Gene Names: Fzd1; Fz1; FZ-1; AW227548
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Frizzled-1 / FZD1; Recombinant Mouse Frizzled-1 / FZD1 Protein (His tag); AW227548; FZ-1; Fzd1; FZD1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 642

• Application Notes: The secreted recombinant mouse FZD1 pro form consists of 191 amino acids and has a predicted molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FZD1 is approximately 35-40 kDa due to glycosylation.
• Predicted N Terminal: Val 69
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FZD1 recombinant protein

Background: Frizzled-1, also known as FZD1, belongs to the G-protein coupled receptor Fz/Smo family. FZD1 contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. FZD1 is expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. Frizzled is a family of G protein-coupled receptor proteins that serve as receptors in the Wnt signaling pathway and other signaling pathways. When activated, Frizzled leads to activation of Dishevelled in the cytosol. Frizzled proteins and the genes encoding them have been identified in an array of animals, from sponges to humans. Frizzled proteins play key roles in governing cell polarity, embryonic development, formation of neural synapses, cell proliferation, and many other processes in developing and adult organisms. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.

Description: A DNA sequence encoding the mouse FZD1 (NP_067432.2) extracellular domain (Met 1-His 248) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31981157
• NCBI GeneID: 14362
• NCBI Accession #: NP_067432.2
• NCBI GenBank Nucleotide #: NM_021457.3
• UniProt Accession #: O70421; O08974; Q7TS82
• Molecular Weight: 71,127 Da
• NCBI Official Full Name: frizzled-1
• NCBI Official Synonym Full Names: frizzled class receptor 1
• NCBI Official Symbol: Fzd1
• NCBI Official Synonym Symbols: Fz1; FZ-1; AW227548
• NCBI Protein Information: frizzled-1
• UniProt Protein Name: Frizzled-1
• Protein Family: Frizzled
• UniProt Gene Name: Fzd1
• UniProt Synonym Gene Names: Fz-1; mFz1

Uniprot Description

FZD1: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5 A1|5 2.61 cM

Cellular Component: cell surface; focal adhesion; integral to membrane; neuron projection; plasma membrane

Molecular Function: frizzled binding; G-protein coupled receptor activity; PDZ domain binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cell-cell signaling; epithelial cell differentiation; hard palate development; negative regulation of BMP signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of protein amino acid phosphorylation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; response to drug; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin