Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) Standard Curve.)
Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit | MOGAT2 elisa kit
Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit
Gene Names
MOGAT2; hDC5; MGAT2; DGAT2L5; DGAT2L5.
Reactivity
Human
Synonyms
2-acylglycerol O-acyltransferase 2 (MOGAT2); Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit; MOGAT2; DGAT2L5; FLJ22644; MGAT2; MGC119183; MGC119184; MGC119185; acyl CoA:monoacylglycerol acyltransferase 2; MOGAT2 elisa kit
Reactivity
Human
Specificity
Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MOGAT2. No significant cross-reactivity or interference between Human MOGAT2 and analogues was observed.
Sequence Length
334
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) Standard Curve.)
Related Product Information for MOGAT2 elisa kit
Description: This Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate MOGAT2 in samples. An antibody specific for MOGAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MOGAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOGAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOGAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MOGAT2 contains a phosphate acyltransferase-like domain and 2 predicted N-linked glycosylation sites. Northern blot analysis revealed expression of 7.2-, 3.0-, and 1.3-kb transcripts in liver, stomach, and small intestine, with lower expression in colon and kidney. A probe specific to the truncated splice variant hybridized mainly to the 1.3-kb transcript in stomach. Immunofluorescence analysis of transiently transfected COS-7 cells showed that MGAT2 displayed a pattern consistent with localization in the endoplasmic reticulum.Substrate specificity assays of MOGAT2 demonstrated that MOGAT2 can catalyze the acylation of rac-1- and sn-2-monooleoylglycerols. MOGAT2 uses a broad range of fatty acyl-CoAs but prefers monoacylglycerols containing unsaturated fatty acids.
Background: MOGAT2 contains a phosphate acyltransferase-like domain and 2 predicted N-linked glycosylation sites. Northern blot analysis revealed expression of 7.2-, 3.0-, and 1.3-kb transcripts in liver, stomach, and small intestine, with lower expression in colon and kidney. A probe specific to the truncated splice variant hybridized mainly to the 1.3-kb transcript in stomach. Immunofluorescence analysis of transiently transfected COS-7 cells showed that MGAT2 displayed a pattern consistent with localization in the endoplasmic reticulum.Substrate specificity assays of MOGAT2 demonstrated that MOGAT2 can catalyze the acylation of rac-1- and sn-2-monooleoylglycerols. MOGAT2 uses a broad range of fatty acyl-CoAs but prefers monoacylglycerols containing unsaturated fatty acids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
2-acylglycerol O-acyltransferase 2
NCBI Official Synonym Full Names
monoacylglycerol O-acyltransferase 2
NCBI Official Symbol
MOGAT2
NCBI Official Synonym Symbols
hDC5; MGAT2; DGAT2L5; DGAT2L5.
NCBI Protein Information
2-acylglycerol O-acyltransferase 2
UniProt Protein Name
2-acylglycerol O-acyltransferase 2
Protein Family
UniProt Gene Name
MOGAT2
UniProt Synonym Gene Names
DC5; DGAT2L5; MGAT2; hMGAT2; hDC5
NCBI Description
The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015]
Uniprot Description
Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
Research Articles on MOGAT2
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Product Notes
The Human MOGAT2 mogat2 (Catalog #AAA9715890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715890 ELISA Kit recognizes Human MOGAT2. It is sometimes possible for the material contained within the vial of "2-acylglycerol O-acyltransferase 2 (MOGAT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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