Typical Testing Data/Standard Curve (for reference only)
Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit | MOGAT2 elisa kit
Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit
Gene Names
MOGAT2; hDC5; MGAT2; DGAT2L5; DGAT2L5.
Reactivity
Human
Synonyms
Monoacylglycerol-O-acyltransferase 2 (MOGAT2); Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit; MGAT2; DGAT2L5; DC5; 2-Acylglycerol O-Acyltransferase 2; Diacylglycerol O-acyltransferase candidate 5; Diacylglycerol acyltransferase 2-like protein 5; MOGAT2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MOGAT2. No significant cross-reactivity or interference between MOGAT2 and analogues was observed.
Sequence Length
334
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOGAT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOGAT2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MOGAT2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MOGAT2 in human tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MOGAT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MOGAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MOGAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MOGAT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MOGAT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MOGAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MOGAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MOGAT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,298 Da
NCBI Official Full Name
2-acylglycerol O-acyltransferase 2
NCBI Official Synonym Full Names
monoacylglycerol O-acyltransferase 2
NCBI Official Symbol
MOGAT2
NCBI Official Synonym Symbols
hDC5; MGAT2; DGAT2L5; DGAT2L5.
NCBI Protein Information
2-acylglycerol O-acyltransferase 2
UniProt Protein Name
2-acylglycerol O-acyltransferase 2
Protein Family
UniProt Gene Name
MOGAT2
UniProt Synonym Gene Names
DC5; DGAT2L5; MGAT2; hMGAT2; hDC5
NCBI Description
The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015]
Uniprot Description
Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
Research Articles on MOGAT2
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Product Notes
The Human MOGAT2 mogat2 (Catalog #AAA450342) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450342 ELISA Kit recognizes Human MOGAT2. It is sometimes possible for the material contained within the vial of "Monoacylglycerol-O-acyltransferase 2 (MOGAT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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