Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit | MOGAT2 elisa kit

Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit

Cat. Num. AAA2020942

• Gene Names: MOGAT2; MGAT2; DGAT2L5
• Reactivity: Human
• Synonyms: Monoacylglycerol-O-acyltransferase 2 (MOGAT2); Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit; MGAT2; DGAT2L5; DC5; 2-Acylglycerol O-Acyltransferase 2; Diacylglycerol O-acyltransferase candidate 5; Diacylglycerol acyltransferase 2-like protein 5; MOGAT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monoacylglycerol-O-acyltransferase 2 (MOGAT2).; No significant cross-reactivity or interference between Monoacylglycerol-O-acyltransferase 2 (MOGAT2) and analogues was observed.
• Sequence Length: 334

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monoacylglycerol-O-acyltransferase 2 (MOGAT2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monoacylglycerol-O-acyltransferase 2 (MOGAT2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MOGAT2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoacylglycerol-O-acyltransferase 2 (MOGAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoacylglycerol-O-acyltransferase 2 (MOGAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoacylglycerol-O-acyltransferase 2 (MOGAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monoacylglycerol-O-acyltransferase 2 (MOGAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MOGAT2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 74355590
• NCBI GeneID: 80168
• UniProt Accession #: Q3SYC2; Q3SYC1; Q6ZQZ2; Q86UH6; Q9H630; A8K7I3
• Molecular Weight: 32,298 Da
• NCBI Official Full Name: Monoacylglycerol O-acyltransferase 2
• NCBI Official Synonym Full Names: monoacylglycerol O-acyltransferase 2
• NCBI Official Symbol: MOGAT2
• NCBI Official Synonym Symbols: MGAT2; DGAT2L5
• NCBI Protein Information: 2-acylglycerol O-acyltransferase 2
• UniProt Protein Name: 2-acylglycerol O-acyltransferase 2
• Protein Family: 2-acylglycerol O-acyltransferase
• UniProt Gene Name: MOGAT2
• UniProt Synonym Gene Names: DC5; DGAT2L5; MGAT2; hMGAT2; hDC5
• UniProt Entry Name: MOGT2_HUMAN

NCBI Description

Dietary fat absorption from the small intestine is facilitated by acyl-CoA:monoacylglycerol transferase (MOGAT; EC 2.3.1.22) and acyl-CoA:diacylglycerol acyltransferase (DGAT; see MIM 604900) activities. MOGAT catalyzes the joining of monoacylglycerol and fatty acyl-CoAs to form diacylglycerol (Yen and Farese, 2003 [PubMed 12621063]).[supplied by OMIM, Mar 2008]

Uniprot Description

MOGAT2: Catalyzes the formation of diacylglycerol from 2- monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity. Belongs to the diacylglycerol acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.22; Membrane protein, integral; Membrane protein, multi-pass; Acetyltransferase

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 2-acylglycerol O-acyltransferase activity; acetyltransferase activity

Biological Process: glycerol metabolic process; diacylglycerol biosynthetic process; triacylglycerol biosynthetic process; intestinal absorption