Delta-like 1 Active Protein | DLL3 active protein

Delta-like 1, Recombinant, Human (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Cat. Num. AAA636764

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Delta-like 1; Recombinant; Human (DLL1; Delta1; DL1; H-Delta-1; UNQ146/PRO172); DLL3 active protein

• Reactivity: Human
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in Tris and NaCl. Reconstitute with sterile PBS to 0.5 mg/ml.

• Activity: Measured by the ability of immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in MC3T3-E1 cells (Nobta M, et al. 2005. J. Biol. Chem. 280:15842). The ED50 for this effect is typically 0.25-1.0ug/ml.
• Endotoxin Level: <1EU by LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, aliquot and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for DLL3 active protein

Delta-like protein 1 (DLL1) is a 90-100kD type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60kD ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

Product Categories/Family for DLL3 active protein

Molecular Biology; MB-Notch Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 8393264
• NCBI GeneID: 10683
• NCBI Accession #: NP_058637.1
• NCBI GenBank Nucleotide #: NM_016941.3
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 57 and 43kD
• NCBI Official Full Name: delta-like protein 3 isoform 1
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3; delta3; drosophila Delta homolog 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3
• UniProt Entry Name: DLL3_HUMAN

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: paraxial mesoderm development; compartment specification; Notch signaling pathway; somitogenesis; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive